Figure 8

Peroxicretion of putative peroxisomal proteins. SDS-PAGE analysis of shake-flask samples of the control strains (panel A) and peroxicreting strains (panel B), peroxicreting different intracellular A. niger enzymes as indicated. When indicated an SKL sequence was added to the C terminus of the protein. After SDS-PAGE the cells were stained with coomassie brilliant blue. Endogenous amylases are indicated. The strains were grown for four days at 30°C, 250 rpm In MEAM. Supernatant was isolated and loaded on a Nupage gel. Lane 1: strong similarity to catalase/peroxidase CpeB, 84 kDa (An01g01830), lane 2: strong similarity to chitinase 1 precursor Cts1, 48 kDa (An02g07020) an SKL sequence was added to the C terminus of the protein, lane 3: strong similarity to alpha-amylase precursor AmyA, 60 kDa (An09g03100) an SKL sequence was added to the C terminus of the protein, lane 4: strong similarity to lipase LipP, 37 kDa (An09g06390) an SKL sequence was added to the C terminus of the protein, lane 5: similarity to endo-1,4-beta-xylanase XynD, 35 kDa (An11g03120) an SKL sequence was added to the C terminus of the protein, lane 6: strong similarity to D-amino acid oxidase Dao1, 41 kDa (An14g05380) an SKL sequence was added to the C terminus of the protein, lane 7: show strong similarity to several fungal alcohol oxidases, 74 kDa (An18g05480).