Figure 4
From: Peroxicretion: a novel secretion pathway in the eukaryotic cell
Expression of the fusion peptide SncA-PmpA. Cell free extracts were obtained from untransformed A. niger (lane 1) and from SncA-PmpA transformed A. niger (lane 2). The Cell free extracts were subjected to SDS-PAGE and western blotting according the manufacturers instructions (Invitrogen) and detection was performed using a custom made antibody against SncA protein (Eurogentec). The expected size of the SncA-PmpA fusion protein is 35 kDa.