Figure 12
From: Peroxicretion: a novel secretion pathway in the eukaryotic cell
Ceramide stimulates peroxicretion, overexpression of PEX11 ortholog (An11g02590) does not. 10 μl of supernatant after 48 hours growth of the strains transformed with the indicated constructs at 30°C, 250 rpm in MEAM. Lane 1; eGFP-SKL, lane 2; eGFP-SKL and C2 ceramide, lane 3; eGFP-SKL (duplo of lane 1), lane 4; MW, lane 5; eGFP-SKL and SncA-PmpA, lane 6; eGFP-SKL, C2 ceramide and SNCA-PMPA, lane 7; eGFP-SKL, SncA-PmpA and PEX11 ortholog, lane 8; eGFP-SKL, C2 ceramide, SncA-PmpA and PEX11 ortholog lane 9; WT, lane 10; WT and C2 ceramide.