Figure 10

Survey of hyphal cells showing the position of a peroxisome (arrow) in the vicinity of the cell membrane in SncA-PmpA expressing A. niger. Panel A. Lengthwise overview of cellular membranes in SncA-PmpA expressing A. niger. Arrow indicates continuity of peroxisomes with the cell membrane (high magnification in panel A1). Panel B. Crosswise overview of cellular membranes in SncA-PmpA expressing A. niger. Arrow indicates continuity of peroxisomes with the cell membrane (high magnification in panel B1). Panel C. Schematic representation of the release of the peroxisomal content due to fusion of the peroxisome with the plasmamembrane. The target SNARE (Sso1 ortholog) is shown in yellow as a transmembrane protein. The chimeric protein SncA-PmpA is depicted in blue (PmpA part) and red (SncA part). For simplicity Sec9 ortholog is not shown. The release of peroxisomal content is depicted, demonstrating peroxicretion of SKL tagged proteins (in green). Panel D. Detail of a glucose-grown A. niger WT cell, showing the presence of peroxisomes that are randomly scattered in the cytosol. M-mitochondrion, N – nucleus, P – peroxisomes, V-vacuole.