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Table 6 QPCR analyses of candidate target mRNAs of EGS-35

From: Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans

Candidate target Primer for QPCR Inhibition level
NM_060051.2 5'-AGTCCGGTTTACTCCAAAGCAA-3' 64%
  5'-CCATGAGGCTTTCCAAATGC-3'  
NM_076865.3 5'-TGGCGTTGCAGATAGAATAGGA-3' 8%
  5'-GCCGAAAGCGACATAACCA-3'  
NM_062956.2 5'-CACCAGTAACCCAACAACTCCTAAA-3' 6 ± 5%
  5'-AAGGAGAATGCGACTGGGAAG-3'  
NM_066665.3 5'-TTGAGGCGAAATGCTTGTCA-3' 7%
  5'-TGATGGCAAAATCGATGCA-3'  
NM_001029738.1 5'-TCGGAAACCAGGCAAACAAC-3' 5%
  5'-GGTCATTGTGTGCCATTTCCTT-3'  
NM_072205.2 5'-TTGGTTAGAAGCGAAGTGAGTGA-3' 9%
  5'-AAGGGAGGAGGAAATCAAGAGG-3'  
NM_061028.1 5'-AGAGCACACGGCACATAGGA-3' 5 ± 5%
  5'-CTTGTTCGGGTCTGGGTTG-3'  
NM_015227.4 5'-GCACCTCAGTCTCAACATTTTCTTT-3' 7%
  5'-TCACACGCCTTCTCTTGGTCT-3'  
NM_001047396.2 5'-GCTCCGATTCAAGTCATGTGG-3' 7 ± 5%
  5'-GCAAGCCGAAGAGGTGATGT-3'  
NM_001047395.1 5'-AGGAACACCAATGGTCACAATG-3' 7%
  5'-GGAACTCCGAGAGCGTAAAGCT-3'  
NM_074332.2 5'-TCGTTCTGTCACGGGGAAC-3' 5 ± 6%
  5'-CTTCGCATCTTTTCCACCAAC-3'  
NM_068962.3 5'-CATCGTCATCTAGTCTCCCAGTGT-3' 8%
  5'-TTACTTCGTTTGGTTGGTGGTG-3'  
  1. The accession number refers to the GenBank database. The values shown are means derived from triplicate experiments, and values for the standard deviation that were less than 5% are not shown.