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Figure 8 | BMC Biotechnology

Figure 8

From: Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans

Figure 8

Reverse genetic screen of C. elegans based on EGS. (A) The flow chart of EGS-clone preparation. (B) The PCR product of the EGS clone IVTT (lanes 1–7). The arrow indicates the 750-bp DNA band (lane M). (C) The transcription product of the EGS clone (lanes 1–7). The arrow indicates the 100-bp RNA band (lane M). (D) Diagram of the phenotype screening procedure.

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