Reverse genetic screen of C. elegans based on EGS. (A) The flow chart of EGS-clone preparation. (B) The PCR product of the EGS clone IVTT (lanes 1–7). The arrow indicates the 750-bp DNA band (lane M). (C) The transcription product of the EGS clone (lanes 1–7). The arrow indicates the 100-bp RNA band (lane M). (D) Diagram of the phenotype screening procedure.