Examination of composition of the EGS library. (A) HincII digestion patterns of the pET28a-EGS clone (lane RV1) containing one HincII site, the pET28a-EGS-clone (lane RV2) containing two or three HincII sites, and pET28a (lane V). The arrow indicates the 5-kb DNA band (lane M). (B) The HincII digestion products of the pET28a-EGS clones containing one HincII site (lanes 1–42) and pET28a (lane V). The arrows indicate the 5-kb DNA band (lane M). (C) The alignment of sequences of some EGS cassettes.