Figure 6From: Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegansDemonstration of FLEGSp and RLEGSp. The partially randomized oligonucleotides of FLESp and RLEGSp are composed of two parts. One is used to amplify pET28a-D, which is equal to pET28a but does not contain the fragment between the T7 terminator and T7 promoter. The other is used to amplify the EGS library cassette.Back to article page