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Figure 2 | BMC Biotechnology

Figure 2

From: Generation of an external guide sequence library for a reverse genetic screen in Caenorhabditis elegans

Figure 2

Effects of EGS on GFP fluorescence of PD4251 worms. (A) Worms were treated with soaking buffer. (B, D, F) Worms were treated with disable EGSs (EGS-Ngfp-lacZ-D (B), EGS-Mtgfp-D (D), or a mix of EGS-Ngfp-lacZ-D and EGS-Mtgfp-D (F)). (C, E, G) Worms were treated with EGSs (EGS-Ngfp-lacZ (C), EGS-Mtgfp (E), or a mix of EGS-Ngfp-lacZ and EGS-Mtgfp (G)). To locate the nuclei, worms were stained with Hoechst 33258 stain.

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