Figure 1
EGSs targeted to Ngfp-lacZ and Mtgfp mRNA. (A) The looped region of "auuuaaauuuuc" was chosen as a target region for EGS-Ngfp-lacZ. (B) The looped region of "guuaauagaauc" was chosen as a target region for EGS-Mtgfp. (C) The "3/4 EGS" derived form was a precursor of tRNATyr in Escherichia coli. (D, E) Complex between Ngfp-lacZ mRNA and EGS-Ngfp-lacZ (D) or EGS-Ngfp-lacZ-D (E). The arrow indicates the site of cleavage by RNase P. EGS-Ngfp-lacZ-D is derived from EGS-Ngfp-lacZ by introducing base-substitution mutations at three positions (highlighted by circles) in the T-loop. (F, G) Complexes between the Mtgfp mRNA and EGS-Mtgfp or EGS-Mtgfp-D, respectively. The arrow indicates the site of RNase P cleavage. EGS-Mtgfp-D is derived from EGS-Mtgfp by introducing base-substitution mutations at three positions (highlighted by circles) of the T-loop.