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Table 1 Kinetic parameters of the interaction between PAI-2 ΔCD-loop or wild-type PAI-2 and VLDLR, both alone and in complex with uPA.

From: The CD-loop of PAI-2 (SERPINB2) is redundant in the targeting, inhibition and clearance of cell surface uPA activity

Analyte Binding Model ka
(M-1 s-1)
kd
(s-1)
KD
(nM)
χ2
PAI-2 wild-type No binding - - - -
uPA:PAI-2 wild-type 1:1 3.75 × 105
(± 1.1 × 105)
1.44 × 10-3
(± 0.39 × 10-3)
4.81
(± 0.64)
4.71
PAI-2 ΔCD-loop No binding - - - -
uPA:PAI-2 ΔCD-loop 1:1 4.16 × 105
(± 1.8 × 105)
1.36 × 10-3
(± 0.23 × 10-3)
4.34
(± 0.56)
3.28
  1. Values were determined using surface plasmon resonance. Binding data was fitted using the BIAevaluation 4.0 software. The binding model chosen represents that with the lowest χ2 value. (Values are the average ± SEM, n = 3).