Table 1 Kinetic parameters of the interaction between PAI-2 ΔCD-loop or wild-type PAI-2 and VLDLR, both alone and in complex with uPA.
Analyte | Binding Model | ka (M-1 s-1) | kd (s-1) | KD (nM) | χ2 |
|---|---|---|---|---|---|
PAI-2 wild-type | No binding | - | - | - | - |
uPA:PAI-2 wild-type | 1:1 | 3.75 × 105 (± 1.1 × 105) | 1.44 × 10-3 (± 0.39 × 10-3) | 4.81 (± 0.64) | 4.71 |
PAI-2 ΔCD-loop | No binding | - | - | - | - |
uPA:PAI-2 ΔCD-loop | 1:1 | 4.16 × 105 (± 1.8 × 105) | 1.36 × 10-3 (± 0.23 × 10-3) | 4.34 (± 0.56) | 3.28 |