Clonea | IGF-E5b production | SD (N = 2) | Rankc production | Totald Fluorescence. | Ranke Fluorescence |
|---|
L | 1.00 | 0.16 | 1 | 1.00 | 1 |
V | 0.46 | 0.10 | 2 | 0.90 | 2 |
A | 0.24 | 0.04 | 3 | 0.30 | 3 |
W | 0.12 | 0.01 | 4 | 0.28 | 4 |
F | 0.10 | 0.02 | 5 | 0.26 | 5 |
J | 0.07 | 0.01 | 6 | 0.17 | 6 |
E | 0.08 | 0.00 | 6 | 0.11 | 7 |
- aNames of stable CHO clones secreting various levels of IGF-E5 (Fig. 3A).
- bProduction from a 3-day batch culture at 30°C was measured by quantitative spot blot against purified IGF-E5. The production was normalized to the highest producer (clone L).
- cRank for IGF-E5 production (column 2) in decreasing order. Clones J and E were both assigned rank 6, because their production level was not significantly different.
- dFluorescence was measured by scanning the wells at 510 nm using the CellCelector™. The fluorescence signal of all the colonies above background was summed and normalized to the highest value (clone L).
- eRank for the total fluorescence (column 5) in decreasing order and independently from column 4.
