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Table 1 Correlation between cell colony fluorescence and recombinant protein secretion from CHO-IGF-E5 clones selected by FLSSM.

From: Fluorescent labeling in semi-solid medium for selection of mammalian cells secreting high-levels of recombinant proteins

Clonea IGF-E5b
production
SD
(N = 2)
Rankc
production
Totald
Fluorescence.
Ranke
Fluorescence
L 1.00 0.16 1 1.00 1
V 0.46 0.10 2 0.90 2
A 0.24 0.04 3 0.30 3
W 0.12 0.01 4 0.28 4
F 0.10 0.02 5 0.26 5
J 0.07 0.01 6 0.17 6
E 0.08 0.00 6 0.11 7
  1. aNames of stable CHO clones secreting various levels of IGF-E5 (Fig. 3A).
  2. bProduction from a 3-day batch culture at 30°C was measured by quantitative spot blot against purified IGF-E5. The production was normalized to the highest producer (clone L).
  3. cRank for IGF-E5 production (column 2) in decreasing order. Clones J and E were both assigned rank 6, because their production level was not significantly different.
  4. dFluorescence was measured by scanning the wells at 510 nm using the CellCelector™. The fluorescence signal of all the colonies above background was summed and normalized to the highest value (clone L).
  5. eRank for the total fluorescence (column 5) in decreasing order and independently from column 4.