Gene therapy by electroporation for the treatment of chronic renal failure in companion animals

Table 6 Hematological parameters in plasmid-GHRH treated cats with CRF.

Assessment			Red Blood Cells	Hematocrit	Hemoglobin	Mean Red Cell Hemoglobin
Control	Day 0	Average score n	7.19 ± .4 n = 12	31.68 ± 1.5 n = 13	11.02 ± .5 n = 12	15.44 ± .4 n = 12
	Day 20	Average score % change P-value vs. Day 0	7.72 ± .5 7.3% P < 0.16 n = 7	32.87 ± 2.0 3.8% P < 0.30 n = 10	10.75 ± .8 -2.0% P < 0.35 n = 8	14.27 ± .6 -8.0% P < 0.06 n = 7
	Day 40	Average score % change P-value vs. Day 0	7.36 ± .6 2.3% P < 0.41 n = 8	32.17 ± 2.0 1.6% P < 0.38 n = 10	10.27 ± .9 -7.0% P < 0.05 n = 9	13.81 ± .7 -11.0% P < 0.03 n = 8
	Day 60–75	Average score % change P-value vs. Day 0	7.40 ± .3 2.9% P < 0.47 n = 7	32.10 ± 1.1 1.3% P < 0.32 n = 7	10.99 ± .5 -0.0% P < 0.25 n = 7	14.9 ± .6 -4.0% P < 0.08 n = 7
Treated	Day 0	Average score n	6.16 ± 0.3 n = 30	27.59 ± 1.1 n = 30	9.65 ± 0.7 n = 30	14.73 ± 0.2 n = 30
	Day 20	Average score % change P-value vs. Day 0	6.71 ± 0.3 9.0% P < 0.001 n = 29	30.46 ± 1.1 10.4% P < 0.0009 n = 29	10.11 ± 0.4 4.7% P < 0.22 n = 29	15.22 ± 0.3 3.3% P < 0.003 n = 29
	Day 40	Average score % change P-value vs. Day 0	6.35 ± 0.3 3.2% P < 0.06 n = 26	28.23 ± 1.1 2.3% P < 0.16 n = 26	9.65 ± 0.3 -0.07% P < 0.47 n = 26	15.32 ± 0.3 4.0% P < 0.001 n = 26
	Day 60–75	Average score % change P-value vs. Day 0	6.42 ± 0.4 4.3% P < 0.02 n = 19	29.18 ± 1.3 5.7% P < 0.03 n = 20	9.82 ± 0.5 1.6% P < 0.002 n = 19	15.46 ± 0.4 4.9% P < 0.004 n = 19

Hematological parameters are significantly improved with plasmid treatment compared to day 0. For cats normal RBC 5.7–10.5 × 10⁶/mm³; normal Ht 38–52%; and normal Hb 9–16 g/dl.