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Table 5 Hematological parameters in plasmid-GHRH treated dogs with CRF.

From: Gene therapy by electroporation for the treatment of chronic renal failure in companion animals

Assessment Red Blood Cells Hematocrit Hemoglobin Mean Red Cell Hemoglobin
Control Day 0 Average score n 6.29 ± 0.5 n = 9 39.98 ± 2.6 n = 12 13.73 ± 1.0 n = 12 23.13 ± 0.8 n = 10
  Day 20 Average score % change P-value vs. Day 0 6.43 ± 0.5 2.2% P < 0.4 n = 6 39.42 ± 1.4 -1.4% P < 0.2 n = 9 13.89 ± 0.8 1.1% P < 0.2 n = 7 22.62 ± 0.7 -2.2% P < 0.05 n = 6
  Day 40 Average score % change P-value vs. Day 0 5.53 ± 0.4 -12% P < 0.3 n = 6 37.82 ± 2.2 -5.4% P < 0.3 n = 6 12.8 ± 1.0 -7.0% P < 0.4 n = 6 23.08 ± 1.1 -0.2% P < 0.26 n = 6
  Day 60–75 Average score % change P-value vs. Day 0 5.69 ± 0.2 -10.0% P < 0.5 n = 2 39.75 ± 1.2 -0.6% P < 0.5 n = 2 12.1 ± 2.2 -12.0% P < 0.5 n = 2 21.1 ± 3.1 -8.8% P < 0.19 n = 2
Treated Day 0 Average score n 4.84 ± 0.3 n = 30 34.95 ± 1.9 n = 30 11.48 ± 0.6 n = 30 23.88 ± 0.2 n = 30
  Day 20 Average score % change P-value vs. Day 0 5.26 ± 0.3 8.6% P < 0.02 n = 27 37.4 ± 1.8 7.0% P < 0.1 n = 27 12.74 ± 0.6 10.9% P < 0.01 n = 27 24.39 ± 0.3 2.1% P < 0.02 n = 27
  Day 40 Average score % change P-value vs. Day 0 5.13 ± 0.3 5.9% P < 0.09 n = 26 36.73 ± 2.1 5.1% P < 0.21 n = 26 12.53 ± 0.6 9.1% P < 0.01 n = 26 24.58 ± 0.3 2.9% P < 0.001 n = 26
  Day 60–75 Average score % change P-value vs. Day 0 5.19 ± 0.3 7.1% P < 0.15 n = 20 37.07 ± 2.2 6% P < 0.25 n = 20 12.69 ± 0.8 9.1% P < 0.02 n = 20 24.31 ± 0.4 1.8% P < 0.1 n = 19
  1. Hematological parameters are significantly improved with plasmid treatment compared to day 0. For dogs normal RBC 5.5–8.5 × 106/mm3; normal Ht 37–55%; and normal Hb 12–18 g/dl.