Gene therapy by electroporation for the treatment of chronic renal failure in companion animals

Table 5 Hematological parameters in plasmid-GHRH treated dogs with CRF.

Assessment			Red Blood Cells	Hematocrit	Hemoglobin	Mean Red Cell Hemoglobin
Control	Day 0	Average score n	6.29 ± 0.5 n = 9	39.98 ± 2.6 n = 12	13.73 ± 1.0 n = 12	23.13 ± 0.8 n = 10
	Day 20	Average score % change P-value vs. Day 0	6.43 ± 0.5 2.2% P < 0.4 n = 6	39.42 ± 1.4 -1.4% P < 0.2 n = 9	13.89 ± 0.8 1.1% P < 0.2 n = 7	22.62 ± 0.7 -2.2% P < 0.05 n = 6
	Day 40	Average score % change P-value vs. Day 0	5.53 ± 0.4 -12% P < 0.3 n = 6	37.82 ± 2.2 -5.4% P < 0.3 n = 6	12.8 ± 1.0 -7.0% P < 0.4 n = 6	23.08 ± 1.1 -0.2% P < 0.26 n = 6
	Day 60–75	Average score % change P-value vs. Day 0	5.69 ± 0.2 -10.0% P < 0.5 n = 2	39.75 ± 1.2 -0.6% P < 0.5 n = 2	12.1 ± 2.2 -12.0% P < 0.5 n = 2	21.1 ± 3.1 -8.8% P < 0.19 n = 2
Treated	Day 0	Average score n	4.84 ± 0.3 n = 30	34.95 ± 1.9 n = 30	11.48 ± 0.6 n = 30	23.88 ± 0.2 n = 30
	Day 20	Average score % change P-value vs. Day 0	5.26 ± 0.3 8.6% P < 0.02 n = 27	37.4 ± 1.8 7.0% P < 0.1 n = 27	12.74 ± 0.6 10.9% P < 0.01 n = 27	24.39 ± 0.3 2.1% P < 0.02 n = 27
	Day 40	Average score % change P-value vs. Day 0	5.13 ± 0.3 5.9% P < 0.09 n = 26	36.73 ± 2.1 5.1% P < 0.21 n = 26	12.53 ± 0.6 9.1% P < 0.01 n = 26	24.58 ± 0.3 2.9% P < 0.001 n = 26
	Day 60–75	Average score % change P-value vs. Day 0	5.19 ± 0.3 7.1% P < 0.15 n = 20	37.07 ± 2.2 6% P < 0.25 n = 20	12.69 ± 0.8 9.1% P < 0.02 n = 20	24.31 ± 0.4 1.8% P < 0.1 n = 19

Hematological parameters are significantly improved with plasmid treatment compared to day 0. For dogs normal RBC 5.5–8.5 × 10⁶/mm³; normal Ht 37–55%; and normal Hb 12–18 g/dl.