Figure 5From: A modified and automated version of the 'Fluorimetric Detection of Alkaline DNA Unwinding' method to quantify formation and repair of DNA strand breaksTime course of DNA strand break repair of Jurkat cells in intervals of 5 min. Cells were X-irradiated on ice with 6.8 Gy (basal line). To measure DNA strand break repair, the damaged cells were incubated at 37°C for different time periods as indicated (grey columns). The black column represents level of SybrGreen fluorescence obtained in undamaged cells (P0 values). Error bars represent SDs from 3 replicates.Back to article page