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Table 2 Summary of rare codon usage in full length native genes.

From: Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer

FtsZ FL native

 

AA

codon

number (%)

Arg

AGG

0

Arg

CGA

1 (6%)

Arg

CGG

0

Gly

GGA

12 (31%)

Gly

GGG

3 (8%)

Leu

CTA

2 (7%)

Pro

CCC

0

Ser

AGT

1 (6%)

NS5B FL native

 

AA

codon

number (%)

Arg

AGG

8 (21%)

Arg

CGA

7 (18%)

Arg

CGG

7 (18%)

Gly

GGA

6 (17%)

Gly

GGG

11 (31%)

Leu

CTA

9 (14%)

Pro

CCC

13 (42%)

Ser

AGT

1 (2%)

P38α FL native

 

AA

codon

number (%)

Arg

AGG

6 (30%)

Arg

CGA

1 (5%)

Arg

CGG

3 (15%)

Gly

GGA

4 (22%)

Gly

GGG

3 (17%)

Leu

CTA

3 (7%)

Pro

CCC

4 (22%)

Ser

AGT

1 (4%)

  1. Codons rarely found in E. coli highly expressed genes are listed on the left. The number of occurrences of each rare codon in the wild type FtsZ, NS5B and P38α full length genes are listed. The percentage of occurrence (number of occurrences/total number of codons for each amino acid) is listed in parentheses.