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Table 2 Summary of rare codon usage in full length native genes.

From: Combined protein construct and synthetic gene engineering for heterologous protein expression and crystallization using Gene Composer

FtsZ FL native  
AA codon number (%)
Arg AGG 0
Arg CGA 1 (6%)
Arg CGG 0
Gly GGA 12 (31%)
Gly GGG 3 (8%)
Leu CTA 2 (7%)
Pro CCC 0
Ser AGT 1 (6%)
NS5B FL native  
AA codon number (%)
Arg AGG 8 (21%)
Arg CGA 7 (18%)
Arg CGG 7 (18%)
Gly GGA 6 (17%)
Gly GGG 11 (31%)
Leu CTA 9 (14%)
Pro CCC 13 (42%)
Ser AGT 1 (2%)
P38α FL native  
AA codon number (%)
Arg AGG 6 (30%)
Arg CGA 1 (5%)
Arg CGG 3 (15%)
Gly GGA 4 (22%)
Gly GGG 3 (17%)
Leu CTA 3 (7%)
Pro CCC 4 (22%)
Ser AGT 1 (4%)
  1. Codons rarely found in E. coli highly expressed genes are listed on the left. The number of occurrences of each rare codon in the wild type FtsZ, NS5B and P38α full length genes are listed. The percentage of occurrence (number of occurrences/total number of codons for each amino acid) is listed in parentheses.