Expression of full length and truncated native and Gene Composer engineered genes in E. coli. A, Total soluble protein was resolved by SDS-PAGE and the resulting commassie blue stained gel is shown. Lanes containing B. subtilis FtsZ (full length: 42.8 kDa, truncated: 34.0 kDa), Hepatitis C Virus NS5B (full length: 66.4 kDa, truncated: 64.0 kDa); and human P38α (full length: 43.5 kDa) are indicated. Lanes from Gene Composer engineered genes are indicated (Eng) and compared to native genes (Native). Expression of full length (FL) and truncated forms (tr) proteins is also compared. Negative control experiments showing expression in the absence of inducer (-) is also shown. B, For each sample, the amount of protein observed was divided by the amount of full length native protein and plotted. By definition, FtsZ native FL, NS5B native FL, and P38α native samples have values of 1 and are shown as controls.