Figure 1

Gene Composer software. Comparative sequence alignments and corresponding structural information are organized by the Alignment Viewer of Gene Composer (top portion of panel). Amino acid sequence of Aquifex aeolicus FtsZ used to obtain FtsZ crystal structure (2R6R) is shown; Chain sequence indicates amino acid residues present in the protein constructs and Model sequence indicates amino acid residues that could be modeled from the electron density maps. Protein secondary structural regions are represented by red helices for alpha helical regions, blue arrows for beta-sheet regions, and green squiggles for turns, as defined the respective PDB file format. Thermal B-factors from the PDB file are normalized and represented in red shading levels (light is low and dark is high). The crystal, ligand, and water contacts are all extracted from the PDB file and listed pictorially. The bacillus subtilis FtsZ sequence (target) is shown on the bottom line and is aligned with FtsZ sequences from multiple species (accession codes indicated). Conserved residues are highlighted (green = high; blue = medium; yellow = low), and a consensus sequence is listed in the bottom row of the alignment.