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Table 1 Sequence errors for 6 synthesized genes.

From: Gene Composer: database software for protein construct design, codon engineering, and gene synthesis

Gene Correct Clones Synonymous Changes Non-Synonymous Changes Deletions Other Mutations Totals
FtsZ 4 1 3 16 0 24
FtsZ/cDNA 2 1 2 14 5 24
NS5B 6 1 0 9 8 24
NS5B/cDNA 4 0 0 7 6 17
P38 9 2 4 6 0 21
P38/cDNA 8 1 0 3 0 12
Totals 33
(27%) 		6
(5%) 		9
(7%) 		55
(45%) 		19
(16%) 		122
  1. Multiple clones representing each of the 6 synthesized genes described in this manuscript were sequenced and the types or errors tabulated. On average about 25% of the sequenced clones were correct. Errors resulting from base deletions were seen in about 50% of clones examined, most likely the result of N-minus-1-mer problem with oligonucleotide synthesis wherein despite high efficiency in oligonucleotide synthesis there is a very small amount but nonetheless unavoidable population of oligonucleotides missing one base somewhere within their sequence [4].
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BMC Biotechnology

ISSN: 1472-6750

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