Gene Composer: database software for protein construct design, codon engineering, and gene synthesis

BMC Biotechnology

Table 1 Sequence errors for 6 synthesized genes.

Gene	Correct Clones	Synonymous Changes	Non-Synonymous Changes	Deletions	Other Mutations	Totals
FtsZ	4	1	3	16	0	24
FtsZ/cDNA	2	1	2	14	5	24
NS5B	6	1	0	9	8	24
NS5B/cDNA	4	0	0	7	6	17
P38	9	2	4	6	0	21
P38/cDNA	8	1	0	3	0	12
Totals	33 (27%)	6 (5%)	9 (7%)	55 (45%)	19 (16%)	122

Multiple clones representing each of the 6 synthesized genes described in this manuscript were sequenced and the types or errors tabulated. On average about 25% of the sequenced clones were correct. Errors resulting from base deletions were seen in about 50% of clones examined, most likely the result of N-minus-1-mer problem with oligonucleotide synthesis wherein despite high efficiency in oligonucleotide synthesis there is a very small amount but nonetheless unavoidable population of oligonucleotides missing one base somewhere within their sequence [4].

ISSN: 1472-6750