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Table 2 List of the double-stranded DNA fragments used to target the mutated genes

From: A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair

Name Size Sequence
200 dsDNA Ctr 195 bp 5'-CGGCA..//..TGGCAGAAGCTATGAAACGATAGGGGCTGAATACAAATCACAGAA..//..TGTTG-3'
3'-GCCGT..//..ACCGTCTTCGATACTTTGCTATCCCCGACTTATGTTTAGTGTCTT..//..CAAGC-5'
500 dsDNA Ctr 494 bp 5'-CACGT..//..TGGCAGAAGCTATGAAACGATAGGGGCTGAATACAAATCACAGAA..//..GTTCG-3'
3'-GTGCA..//..ACCGTCTTCGATACTTTGCTATCCCCGACTTATGTTTAGTGTCTT..//..CAAGC-5'
1000 dsDNA Ctr 980 bp 5'-TACAA..//..TGGCAGAAGCTATGAAACGATAGGGGCTGAATACAAATCACAGAA..//..AGATT-3'
3'-ATGTT..//..ACCGTCTTCGATACTTTGCTATCCCCGACTTATGTTTAGTGTCTT..//..TCTAA-5'
2200 dsDNA Ctr 2133 bp 5'-AAATG..//..TGGCAGAAGCTATGAAACGATAGGGGCTGAATACAAATCACAGAA..//..ACATT-3'
3'-TTTAC..//..ACCGTCTTCGATACTTTGCTATCCCCGACTTATGTTTAGTGTCTT..//..TGTAA-5'
200 dsDNA Rep 195 bp 5'-CGGCA..//..TGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAA..//..TGTTG-3'
3'-GCCGT..//..ACCGTCTTCGATACTTTGCTATACCCGACTTATGTTTAGTGTCTT..//..CAAGC-5'
500 dsDNA Rep 494 bp 5'-CACGT..//..TGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAA..//..GTTCG-3'
3'-GTGCA..//..ACCGTCTTCGATACTTTGCTATACCCGACTTATGTTTAGTGTCTT..//..CAAGC-5'
1000 dsDNA Rep 980 bp 5'-TACAA..//..TGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAA..//..AGATT-3'
3'-ATGTT..//..ACCGTCTTCGATACTTTGCTATACCCGACTTATGTTTAGTGTCTT..//..TCTAA-5'
2200 dsDNA Rep 2133 bp 5'-AAATG..//..TGGCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAGAA..//..ACATT-3'
3'-TTTAC..//..ACCGTCTTCGATACTTTGCTATACCCGACTTATGTTTAGTGTCTT..//..TGTAA-5'
GFP 700 dsDNA Ctr 732 bp 5'-AAATG..//..CAAGCTGCCCGTGCCCTAGCCAACCCTCGTGACCA..//..GAGCA-3'
3'-TTTAC..//..GTTCGACGGGCACGGGATCGGTTGGGAGCACTGGT..//..CTCGT-5'
GFP 700 dsDNA Rep 732 bp 5'-AAATG..//..CAAGCTGCCCGTGCCCTGGCCAACCCTCGTGACCA..//..GAGCA-3'
3'-TTTAC..//..GTTCGACGGGCACGGGACCGGTTGGGAGCACTGGT..//..CTCGT-5'
  1. Ctr, non-correcting control fragment; Rep, repair fragment; GFP, green fluorescent protein; in bold, position of the base to correct.