Table 1 List of the single-stranded oligodeoxynucleotides used to target the mutated genes
Name | Size | Sequence | Characteristics |
|---|---|---|---|
Luc PO AS Ctr | 35 b | 5'-TGATTTGTATTCAGCCCCTATCGTTTCATAGCTTC-3' | Antisense Control phosphodiester ssODN |
Luc PO S Ctr | 35 b | 5'-GAAGCTATGAAACGATAGGGGCTGAATACAAATCA-3' | Sense Control phosphodiester ssODN |
Luc PS AS Ctr | 35 b | 5'-TsGsAsTTTGTATTCAGCCCCTATCGTTTCATAGCsTsTsC-3' | Antisense Control ssODN with 3 PS linkages at each end |
Luc PS S Ctr | 35 b | 5'-GsAsAsGCTATGAAACGATAGGGGCTGAATACAAAsTsCsA-3' | Sense Control ssODN with 3 PS linkages at each end |
Luc PS AS Ctr | 45 b | 5'-TsTsCsTGTGATTTGTATTCAGCCCCTATCGTTTCATAGCTTCTGsCsCsA-3' | Antisense Control ssODN with 3 PS linkages at each end |
Luc PO AS Rep | 35 b | 5'-TGATTTGTATTCAGCCCATATCGTTTCATAGCTTC-3' | Antisense phosphodiester ssODN |
Luc PO S Rep | 35 b | 5'-GAAGCTATGAAACGATATGGGCTGAATACAAATCA-3' | Sense phosphodiester ssODN |
Luc PS AS Rep | 35 b | 5'-TsGsAsTTTGTATTCAGCCCATATCGTTTCATAGCsTsTsC-3' | Antisense ssODN with 3 PS linkages at each end |
Luc PS S Rep | 35 b | 5'-GsAsAsGCTATGAAACGATATGGGCTGAATACAAAsTsCsA-3' | Sense ssODN with 3 PS linkages at each end |
Luc PO AS Rep | 45 b | 5'-TTCTGTGATTTGTATTCAGCCCATATCGTTTCATAGCTTCTGCCA-3' | Antisense phosphodiester ssODN |
Luc PS AS Rep | 45 b | 5'-TsTsCsTGTGATTTGTATTCAGCCCATATCGTTTCATAGCTTCTGsCsCsA-3' | Antisense ssODN with 3 PS linkages at each end |
Luc PO S Rep | 45 b | 5'-TsGsGsCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAsGsAsA-3' | Sense ssODN with 3 PS linkages at each end |
Luc LNA 1.1 AS Rep | 45 b | 5'-TTCTGTGATTTGTATTCAGCCCATATCGTTTCATAGCTTCTGCCA-3' | Antisense ssODN with 1 LNA residue at each end |
Luc LNA 4.4 AS Rep | 45 b | 5'-TTCTGTGATTTGTATTCAGCCCATATCGTTTCATAGCTTCTGCCA-3' | Antisense ssODN with 4 LNA residues at each end |
Luc LNA 4r AS Rep | 45 b | 5'-TTCTGTGATTTGTATTCAGCCCATATCGTTTCATAGCTTCTGCCA-3' | Antisense ssODN with 4 LNA residues distributed over the sequence |
GFP PO AS Ctr | 35 b | 5'-TGGTCACGAGGGTTGGCTAGGGCACGGGCAGCTTG-3' | Antisense Control phosphodiester ssODN |
GFP PS AS Ctr | 35 b | 5'-TsGsGsTCACGAGGGTTGGCTAGGGCACGGGCAGCsTsTsG-3' | Antisense Control ssODN with 3 PS linkages at each end |
GFP PO AS Rep | 35 b | 5'-TGGTCACGAGGGTTGGCCAGGGCACGGGCAGCTTG-3' | Antisense phosphodiester ssODN |
GFP PS AS Rep | 35 b | 5'-TsGsGsTCACGAGGGTTGGCCAGGGCACGGGCAGCsTsTsG-3' | Antisense ssODN with 3 PS linkages at each end |