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Table 1 List of the single-stranded oligodeoxynucleotides used to target the mutated genes

From: A comparison of synthetic oligodeoxynucleotides, DNA fragments and AAV-1 for targeted episomal and chromosomal gene repair

Name Size Sequence Characteristics
Luc PO AS Ctr 35 b 5'-TGATTTGTATTCAGCCCCTATCGTTTCATAGCTTC-3' Antisense Control phosphodiester ssODN
Luc PO S Ctr 35 b 5'-GAAGCTATGAAACGATAGGGGCTGAATACAAATCA-3' Sense Control phosphodiester ssODN
Luc PS AS Ctr 35 b 5'-TsGsAsTTTGTATTCAGCCCCTATCGTTTCATAGCsTsTsC-3' Antisense Control ssODN with 3 PS linkages at each end
Luc PS S Ctr 35 b 5'-GsAsAsGCTATGAAACGATAGGGGCTGAATACAAAsTsCsA-3' Sense Control ssODN with 3 PS linkages at each end
Luc PS AS Ctr 45 b 5'-TsTsCsTGTGATTTGTATTCAGCCCCTATCGTTTCATAGCTTCTGsCsCsA-3' Antisense Control ssODN with 3 PS linkages at each end
Luc PO AS Rep 35 b 5'-TGATTTGTATTCAGCCCATATCGTTTCATAGCTTC-3' Antisense phosphodiester ssODN
Luc PO S Rep 35 b 5'-GAAGCTATGAAACGATATGGGCTGAATACAAATCA-3' Sense phosphodiester ssODN
Luc PS AS Rep 35 b 5'-TsGsAsTTTGTATTCAGCCCATATCGTTTCATAGCsTsTsC-3' Antisense ssODN with 3 PS linkages at each end
Luc PS S Rep 35 b 5'-GsAsAsGCTATGAAACGATATGGGCTGAATACAAAsTsCsA-3' Sense ssODN with 3 PS linkages at each end
Luc PO AS Rep 45 b 5'-TTCTGTGATTTGTATTCAGCCCATATCGTTTCATAGCTTCTGCCA-3' Antisense phosphodiester ssODN
Luc PS AS Rep 45 b 5'-TsTsCsTGTGATTTGTATTCAGCCCATATCGTTTCATAGCTTCTGsCsCsA-3' Antisense ssODN with 3 PS linkages at each end
Luc PO S Rep 45 b 5'-TsGsGsCAGAAGCTATGAAACGATATGGGCTGAATACAAATCACAsGsAsA-3' Sense ssODN with 3 PS linkages at each end
Luc LNA 1.1 AS Rep 45 b 5'-TTCTGTGATTTGTATTCAGCCCATATCGTTTCATAGCTTCTGCCA-3' Antisense ssODN with 1 LNA residue at each end
Luc LNA 4.4 AS Rep 45 b 5'-TTCTGTGATTTGTATTCAGCCCATATCGTTTCATAGCTTCTGCCA-3' Antisense ssODN with 4 LNA residues at each end
Luc LNA 4r AS Rep 45 b 5'-TTCTGTGATTTGTATTCAGCCCATATCGTTTCATAGCTTCTGCCA-3' Antisense ssODN with 4 LNA residues distributed over the sequence
GFP PO AS Ctr 35 b 5'-TGGTCACGAGGGTTGGCTAGGGCACGGGCAGCTTG-3' Antisense Control phosphodiester ssODN
GFP PS AS Ctr 35 b 5'-TsGsGsTCACGAGGGTTGGCTAGGGCACGGGCAGCsTsTsG-3' Antisense Control ssODN with 3 PS linkages at each end
GFP PO AS Rep 35 b 5'-TGGTCACGAGGGTTGGCCAGGGCACGGGCAGCTTG-3' Antisense phosphodiester ssODN
GFP PS AS Rep 35 b 5'-TsGsGsTCACGAGGGTTGGCCAGGGCACGGGCAGCsTsTsG-3' Antisense ssODN with 3 PS linkages at each end
  1. Luc, luciferase; GFP, green fluorescent protein; AS, antisense; S, sense; Ctr, non-correcting control oligonucleotide; Rep, repair oligonucleotide; PO, phosphodiester; PS, phosphorothioate; LNA, locked nucleic acid (underlined); s, phosphorothioate linkage; in bold, position of the base to correct.
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BMC Biotechnology

ISSN: 1472-6750

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