Figure 10

Quantitative PCR: Expression of desmin, MyoD and MEF-2d in collagen-I-fibrin gels. Group 1: desmin was 4-fold up-regulated (± 0,41 cycles), MyoD (21,11-fold ± 0,58) and MEF-2d (8,88-fold ± 0,37) were also clearly up-regulated in relation to neonatal muscle tissue. Group 3: over-expression of MyoD (6,06-fold ± 0,54) and MEF-2d (12,13-fold ± 0,98) but only slight up-regulation of desmin (1,57-fold ± 0,52). Group 2: desmin (0,03-fold ± 0,36) and MyoD (0,16-fold ± 0,29) down-regulated, MEF-2-expression (1,11-fold ± 0,05) was nearly equal to neonatal muscle tissue. Group 4: down-regulation of desmin (0,03-fold ± 0,09) and MyoD (0,03-fold ± 0,92), MEF-2d was nearly 3-fold over-expressed (2,93 ± 0,05).