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Figure 9 | BMC Biotechnology

Figure 9

From: Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and function

Figure 9

The IGF-1 proteins isolated from the IgG-sepharose column were separated on a 2-D focusing/phoresis acrylamide gel. Five μg of the IGF-1 – S. aureus fusion protein was loaded on a 10% bis-acrylamide gel for electrofocusing and phoresis. Electrofocusing was conducted initially over a pH range of 4–10. Following isoelectric focusing and electrophoresis, four proteins (6, 7, 14 and 25 kDa) were detected after Coomassie Blue staining. These proteins corresponded in molecular weight to ZZ-IGF-1 (A), Z-IGF-1 (B) and IGF-1 (C).

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BMC Biotechnology

ISSN: 1472-6750

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