Figure 2From: Optimization of codon composition and regulatory elements for expression of human insulin like growth factor-1 in transgenic chloroplasts and evaluation of structural identity and functionIGF-1 Expression in E. coli. Expression of IGF-1 in western blots was detected using mouse anti-human IGF-1. Lane: 1-untransformed E. coli; Lane 2-pLD5'UTRZZTEVIGF-1n; Lane 3: pLD5'UTRZZTEVIGF-1s. The zz tag-TEV-IGF-1 polypeptide has a molecular size of 24 kDa.Back to article page