Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling

BMC Biotechnology

Table 2 Substitutions made during the creation of E2-Red/Green and E2-Orange.

Construct	Residues differing from DsRed-Express2
E2-Red/Green^a	Q42H, A44V, A217T, A219G
E2-Orange^b	Q66T, V71A^c, S179T^c

^aGenBank Accession Number FJ498892
^bGenBank Accession Number FJ498891
^c These mutations were identified during random mutagenesis. All other mutations resulted from targeted mutagenesis.

ISSN: 1472-6750