E2-Red/Green is a useful third color for flow cytometry. Shown is a dot plot generated by flow cytometry of S. cerevisiae cells expressing DsRed-Express2, E2-Red/Green, EGFP, or no FP. Cells were grown individually, pooled at equivalent cell concentrations, and analyzed by flow cytometry. Fluorescence was excited using a 488-nm laser, and red and green signals were detected using PE and FITC filter sets, respectively. On the right are the same data with DsRed-Express2-expressing cells boxed in red, E2-Red/Green-expressing cells boxed in blue, and EGFP-expressing cells boxed in green.