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Figure 1 | BMC Biotechnology

Figure 1

From: Noncytotoxic orange and red/green derivatives of DsRed-Express2 for whole-cell labeling

Figure 1

Fluorescence properties of E2-Orange. Shown are (A) excitation and emission and (B) absorbance spectra of E2-Orange. (C) Maturation kinetics of E2-Orange fluorescence. For these measurements the FPs were excited at 520 ± 10 nm excitation and emission was recorded at 560 ± 10 nm. (D) Photobleaching kinetics of E2-Orange (black line), mOrange2 (blue line), KO (gray line), and mKO2 (red line) during widefield fluorescence microscopy. Data were collected using a Texas Red filter set. For (C) and (D), data represent the mean of three independent measurements.

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