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Table 1 Karyotypes of primary human fibroblasts

From: PhiC31 integrase induces a DNA damage response and chromosomal rearrangements in human adult fibroblasts

  Cytogenetic analysis
Transfected constructs Transfection experiment 1 Transfection experiment 2
pBabepuro 46, XY (n = 10) 46, XY (n = 15)
pBabepuroatt 46, XY (n = 10) 46, XY (n = 18)
pBabepuro + pCMV-Int 46, XY (n = 6)  
  Extra chr. 10 (n = 1)  
  Del(1)(p11) (n = 1)  
  Add 17q (n = 1)  
  Chr. 2 loss (n = 1)  
pBabepuroatt + pCMV-Int 46, XY (n = 6)  
  Extra chr. (n = 1)  
  Ring Chr. No. 7 (n = 2)  
  Del(13)(q21.1) (n = 1)  
pBabepuro + pCMV-SB   46, XY (n = 17)
pBabepuro + pCMV-mSB   46, XY (n = 2)
pBabepuro-SB*   46, XY (n = 16)
pBabepuro-mSB*   46, XY (n = 15)
  1. Number of cells analysed (n) are indicated in parentheses. *The expression cassettes for SB or mutant SB (mSB) were cloned into the pBabepuro plasmid. Metaphase chromosomes were analyzed by standard Q-banding techniques. The description of chromosome aberrations was based on the recommendations of the International System for Human Cytogenetic Nomenclature (ISCN 1995).