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Cytogenetic analysis
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Transfected constructs
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Transfection experiment 1
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Transfection experiment 2
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pBabepuro
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46, XY (n = 10)
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46, XY (n = 15)
|
|
pBabepuroatt
|
46, XY (n = 10)
|
46, XY (n = 18)
|
|
pBabepuro + pCMV-Int
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46, XY (n = 6)
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| |
Extra chr. 10 (n = 1)
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Del(1)(p11) (n = 1)
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| |
Add 17q (n = 1)
| |
| |
Chr. 2 loss (n = 1)
| |
|
pBabepuroatt + pCMV-Int
|
46, XY (n = 6)
| |
| |
Extra chr. (n = 1)
| |
| |
Ring Chr. No. 7 (n = 2)
| |
| |
Del(13)(q21.1) (n = 1)
| |
|
pBabepuro + pCMV-SB
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46, XY (n = 17)
|
|
pBabepuro + pCMV-mSB
| |
46, XY (n = 2)
|
|
pBabepuro-SB*
| |
46, XY (n = 16)
|
|
pBabepuro-mSB*
| |
46, XY (n = 15)
|
- Number of cells analysed (n) are indicated in parentheses. *The expression cassettes for SB or mutant SB (mSB) were cloned into the pBabepuro plasmid. Metaphase chromosomes were analyzed by standard Q-banding techniques. The description of chromosome aberrations was based on the recommendations of the International System for Human Cytogenetic Nomenclature (ISCN 1995).
