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Table 1 Karyotypes of primary human fibroblasts

From: PhiC31 integrase induces a DNA damage response and chromosomal rearrangements in human adult fibroblasts

 

Cytogenetic analysis

Transfected constructs

Transfection experiment 1

Transfection experiment 2

pBabepuro

46, XY (n = 10)

46, XY (n = 15)

pBabepuroatt

46, XY (n = 10)

46, XY (n = 18)

pBabepuro + pCMV-Int

46, XY (n = 6)

 
 

Extra chr. 10 (n = 1)

 
 

Del(1)(p11) (n = 1)

 
 

Add 17q (n = 1)

 
 

Chr. 2 loss (n = 1)

 

pBabepuroatt + pCMV-Int

46, XY (n = 6)

 
 

Extra chr. (n = 1)

 
 

Ring Chr. No. 7 (n = 2)

 
 

Del(13)(q21.1) (n = 1)

 

pBabepuro + pCMV-SB

 

46, XY (n = 17)

pBabepuro + pCMV-mSB

 

46, XY (n = 2)

pBabepuro-SB*

 

46, XY (n = 16)

pBabepuro-mSB*

 

46, XY (n = 15)

  1. Number of cells analysed (n) are indicated in parentheses. *The expression cassettes for SB or mutant SB (mSB) were cloned into the pBabepuro plasmid. Metaphase chromosomes were analyzed by standard Q-banding techniques. The description of chromosome aberrations was based on the recommendations of the International System for Human Cytogenetic Nomenclature (ISCN 1995).