
Cytogenetic analysis


Transfected constructs

Transfection experiment 1

Transfection experiment 2


pBabepuro

46, XY (n = 10)

46, XY (n = 15)

pBabepuroatt

46, XY (n = 10)

46, XY (n = 18)

pBabepuro + pCMVInt

46, XY (n = 6)
 

Extra chr. 10 (n = 1)
 

Del(1)(p11) (n = 1)
 

Add 17q (n = 1)
 

Chr. 2 loss (n = 1)
 
pBabepuroatt + pCMVInt

46, XY (n = 6)
 

Extra chr. (n = 1)
 

Ring Chr. No. 7 (n = 2)
 

Del(13)(q21.1) (n = 1)
 
pBabepuro + pCMVSB
 
46, XY (n = 17)

pBabepuro + pCMVmSB
 
46, XY (n = 2)

pBabepuroSB*
 
46, XY (n = 16)

pBabepuromSB*
 
46, XY (n = 15)

 Number of cells analysed (n) are indicated in parentheses. *The expression cassettes for SB or mutant SB (mSB) were cloned into the pBabepuro plasmid. Metaphase chromosomes were analyzed by standard Qbanding techniques. The description of chromosome aberrations was based on the recommendations of the International System for Human Cytogenetic Nomenclature (ISCN 1995).