Figure 5

Simultaneous targeting of Oct-4 mRNA and SSEA-1 protein in live P19 cells analyzed by deconvolution microscopy and flow cytometry. A. Deconvolution microscopy imaging of Oct-4 mRNA and SSEA-1 protein. Top panel shows that targeting of SSEA-1 protein using dye-labeled antibody gave strong signal (green) in undifferentiated (UD) cells and very weak signal in RA-treated (differentiated) cells. Random beacons only gave very weak signal (red). Bottom panel shows the fluorescence images of simultaneous targeting of Oct-4 mRNA (red) and SSEA-1 protein (green) in undifferentiated (UD) RA-treated (RA) cells. Scale bar = 15 μM. B. Flow cytometric analysis of signal from targeting Oct-4 mRNA and SSEA-1 protein in undifferentiated (top panel) and differentiated (bottom panel) cells. Right and left panels show respectively the results of cells with and without MB and SSEA-1 antibody. X- and Y-axis show respectively the relative fluorescence intensities of imaging Oct-4 mRNA and SSEA-1 protein.