Figure 4

CD spectra of RNase A and CHMO refolded with the ternary refolding matrix (mini-chaperone, DsbA and hPPIase). The CD spectra refolded RNase A (A) and CHMO (B) were compared with those of native and denatured and reduced RNase A and CHMO, respectively. The CD spectra were obtained using a JASCO J-715 in a quartz cuvette with 1 mm path length. RNase A and CHMO refolded by the ternary refolding gel was dissolved in 25 mM sodium phosphate buffer, pH 7.0 at 0.05 mg/ml.