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Figure 2 | BMC Biotechnology

Figure 2

From: Folding machineries displayed on a cation-exchanger for the concerted refolding of cysteine- or proline-rich proteins

Figure 2

Coupling density of folding machineries to a cation exchanger. Various amounts of purified folding machineries were mixed with the cation exchanger, SP Sepharose Fast Flow. The folding machineries-bound SP Sepharose was mixed with 2 bead volume of 50 mM sodium phosphate buffer, pH 8.4 containing 1 M NaCl. After gentle mixing for 30 min at 25°C, the bead was removed by centrifugation and the supernatant was then used to the determination of protein concentration.

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