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Figure 4 | BMC Biotechnology

Figure 4

From: Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast

Figure 4

Detection of LUXCt protein accumulation in transgenic C. reinhardtii strains. Ten or two micrograms of total proteins from untransformed wild type (WT), rbcL-luxCt fusion transformant (rbcL-luxCt/rbcLwt), combined rbcL-luxCt fusion/rbcL knock-out transformant (rbcL-luxCt/rbcL-), and the luxCt expressing strains, luxCt and psbA-luxCt, were separated by 12% SDS-PAGE and stained with Coomassie blue (left panel), or blotted onto nitrocellulose membranes and decorated with anti-LUXAB (middle panel) or anti-Rubisco (right panel) antibodies. Protein levels from these strains were compared to a strain expressing LUXCt from the p322 integration site. Proteins were visualized on Western blots by alkaline phosphatase activity staining. Proteins were derived from cultures grown on TAP.

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BMC Biotechnology

ISSN: 1472-6750

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