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Figure 3 | BMC Biotechnology

Figure 3

From: Accumulation and processing of a recombinant protein designed as a cleavable fusion to the endogenous Rubisco LSU protein in Chlamydomonas chloroplast

Figure 3

Southern and Northern blot analysis of rbcL-luxCt fusion ( rbcL-luxCt/rbcLwt) and combined rbcL-luxCt fusion endogenous rbcL knock-out ( rbcL-luxCt/rbcL-) strains. Lane 1, untransformed wild type (WT); lane 2, rbcL-luxCt fusion transformant rbcL-luxCt/rbcLwt; lane 3, combined rbcL-luxCt fusion/rbcL knock-out transformant (rbcL-luxCt/rbcL-). A C. reinhardtii DNA was digested with EcoRI and XhoI, and hybridized with the Bam-Xho probe (left panel), BamHI for aphA6 (second panel from the left) or lux probes (third panel from left), and EcoRI and BamHI for rbcL probes (right panel), respectively. B Detection of luxCt and rbcL mRNA expression in transgenic C. reinhardtii transformants. Total RNA isolated from WT, rbcL-luxCt/rbcLwt, and rbcL-luxCt/rbcL- was separated on denaturing agarose gels (left panel) and blotted onto nylon membrane. The membranes were hybridized with luxCt (middle panel) or rbcL (right panel) cDNA probes.

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