Figure 1

Maps of luxCt , rbcL-luxCt/rbcL^wt, and rbcL-luxCt/rbcL^- constructs for expression in C. reinhardtii chloroplasts. A Schematic diagram of the replaced region, including relevant restriction sites. Homologous regions used for recombination between the insertion plasmid and the C. reinhardtii chloroplast genome are shown as flanking genome regions, and insert psbA sites in the chloroplast genome. B Map of the vector targeting the p322 inverted-repeat within the chloroplast. Relevant restriction sites delineate the rbcL 5' UTR (BamHI-NdeI), rbcL and luxCt coding regions, linker and preferredoxin transit peptide (preFd) regions, and the rbcL 3' UTR (XbaI-BamHI). Map showing the homologous region between the p322 plasmid and the C. reinhardtii chloroplast genome into which the chimeric rbcL-luxCt fusion gene was integrated. C. reinhardtii chloroplast DNA is depicted as the EcoRI to XhoI fragment of 5.7-kb located in the inverted repeat region of the chloroplast genome. C Transgene replacement of the endogenous rbcL gene through rbcL 5' and 3' homologous recombination (crossed lines) thereby knocking-out the original rbcL coding region with the aphA6 kanamycin-resistance gene. The thick black lines indicate regions corresponding to the probes used in the Southern and Northern blot analysis.