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Table 1 FLIM measurements for the TagGFP and TagGFP-TagRFP pair

From: Practical and reliable FRET/FLIM pair of fluorescent proteins

Construct n1 τφ [ns]2 τM [ns]3 φ [%]4 M [%]4
TagGFP 20 2.55 ± 0.05 2.64 ± 0.04 - -
TagGFP-TagRFP 23 1.57 ± 0.07 1.98 ± 0.07 38 24
  1. 1n number of cells from which the lifetime was calculated
  2. 2τφ average phase lifetime ± standard deviation
  3. 3τM average modulation lifetime ± standard deviation
  4. 4E average FRET efficiency calculated from τφ or τM