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Table 1 FLIM measurements for the TagGFP and TagGFP-TagRFP pair

From: Practical and reliable FRET/FLIM pair of fluorescent proteins

Construct

n1

τφ [ns]2

Ï„M [ns]3

Eτφ [%]4

EÏ„M [%]4

TagGFP

20

2.55 ± 0.05

2.64 ± 0.04

-

-

TagGFP-TagRFP

23

1.57 ± 0.07

1.98 ± 0.07

38

24

  1. 1n number of cells from which the lifetime was calculated
  2. 2τφ average phase lifetime ± standard deviation
  3. 3τM average modulation lifetime ± standard deviation
  4. 4E average FRET efficiency calculated from τφ or τM
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BMC Biotechnology

ISSN: 1472-6750

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