Figure 1

T-DNA region of the pTRAk plant expression vector and gene cassettes targeting murine and viral IL-10 to different subcellular locations. LB and RB, left and right border of the T-DNA; Pnos and pAnos, promoter and terminator of the nopaline synthase gene; nptII, coding sequence of the neomycin phosphotransferase gene; SAR, scaffold attachment region; P and pA, 35S promoter with duplicated enhancer and terminator of the cauliflower mosaic virus (CaMV) 35S gene; TL, 5' UTR of the tobacco etch virus (TEV); sp, native signal peptide; IL-10, mature IL-10 sequence; His₆, histidine tag; SEKDEL, ER retention signal; TcR, C-terminal hinge and transmembrane domain of the human T-cell receptor; cytb5, transmembrane domain of the rat cytochrome-b5. Not drawn to scale.