Analysis of fibrillar BSA by using ThT and transmission electron microscopy. (A) Comparison of fluorescence levels of increasing concentrations of G-BSA, BSA-S200, BSA-S75 and Aβ25–35 after incubating with 20 μM amyloid-specific dye ThT for 1 h. Amyloid Aβ25–35 that had been dissolved in sterile double-distilled water and aged at 37°C for 3 days was used as a positive control. The fluorescence level of G-BSA was similar to that of BSA-S75. Data are means ± S.D. (n = 3). TEM images show structure of G-BSA (B), BSA-S200 (C), BSA-S75 (D), Aβ25–35 (E), BSA-HW55S (F) and BSA-Zwit (G).