Formation of fibrillar BSA on Superdex-200 column chromatography. (A) BSA (2 mg/ml dissolved in PBS containing 1% SDS) was applied to a Superdex-200 column and eluted at the rate of 1 ml/min with a buffer solution containing 0.05% SDS. (B) Fractions from 23 to 33 were analyzed by SDS-PAGE and stained with Coomassie Blue. Lane A contains G-BSA as control. Before loading to the gel, the fibrillar BSA was solubilized in a sample buffer solution containing 1% SDS and β-mercaptoethanol and boiled for 10 min, which caused the fibrillar BSA to disassemble and migrate to the same position as G-BSA on SDS-PAGE.