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Table 1 Effects of different concentrations of arginine on the refolding recovery yield.

From: An optimised system for refolding of human glucose 6-phosphate dehydrogenase

Added Arg concentration (mM) 0 200 300 400 500 600 700
Refolding yield (%) 2.6 ± 0.1 44.3 ± 3.5 59.4 ± 3.8 69.5 ± 4.1 71.1 ± 4.0 72.2 ± 4.3 72.1 ± 5.1
  1. L-arginine was added into the refolding buffer containing 50 mM NaCl, 200 μM NADP+, 10 mM DTT, 40 μM PEG 3350 in 50 mM Tris, pH 8.0 to reach final concentrations of Arg from 0 to 700 mM. The refolding mixture was incubated at 25°C. Activity was monitored over several days and the final regain of G6PD activity is recorded.