An optimised system for refolding of human glucose 6-phosphate dehydrogenase

BMC Biotechnology

Table 1 Effects of different concentrations of arginine on the refolding recovery yield.

Added Arg concentration (mM)	0	200	300	400	500	600	700
Refolding yield (%)	2.6 ± 0.1	44.3 ± 3.5	59.4 ± 3.8	69.5 ± 4.1	71.1 ± 4.0	72.2 ± 4.3	72.1 ± 5.1

L-arginine was added into the refolding buffer containing 50 mM NaCl, 200 μM NADP⁺, 10 mM DTT, 40 μM PEG 3350 in 50 mM Tris, pH 8.0 to reach final concentrations of Arg from 0 to 700 mM. The refolding mixture was incubated at 25°C. Activity was monitored over several days and the final regain of G6PD activity is recorded.

ISSN: 1472-6750