Figure 5

Gel-elution profile of native G6PD and refolded G6PD by FPLC. Denatured protein was diluted into the refolding buffer containing 50 mM NaCl, 400 mM Arg, 200 μM NADP⁺, and 10 mM DTT in 50 mM Tris, pH 7.5 at 25°C. Refolded protein samples were withdrawn after incubation for 0, 1, 2, 3, 5, and 7 days. 100 μl samples (around 200 μg/ml) of G6PD refolded protein intermediates and the native protein solutions were loaded on a Superdex 200 HR 10/30 column equilibrated with 0.1 M Tris-HCl, 150 mM NaCl, pH 7.6. Apparent molecular weights of refolded G6PD: 203.6 kDa (10.95 ml), 100.1 kDa (12.36 ml), and 50 kDa (13.78 ml).