Figure 4

Effects of temperature and pH on the refolding recovery yield. Denatured protein was diluted into the refolding buffer containing 50 mM NaCl, 400 mM Arg, 200 μM NADP⁺, and 10 mM DTT in 50 mM Tris with pH ranging from 7.5 to 8.5. The refolding mixture was incubated at (a) 25°C, (b) 30°C, and (c) 37°C, respectively and the regained G6PD activity was followed.