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Figure 1 | BMC Biotechnology

Figure 1

From: A novel method for producing target cells and assessing cytotoxic T lymphocyte activity in outbred hosts

Figure 1

Outline of target cell production. Primary feline fibroblasts were immortalized by transfection with plasmid pACTSV2, an SV40 genome encoding a functional small T antigen and an internally deleted large T antigen. The cells thus immortalized were then transduced with vesicular stomatitis virus glycoprotein G (VSV-G) pseudotyped particles produced in 293T cells and delivering target env gene and reporter GFP gene.

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