Table 1 List of primers used in the real-time PCR.
From: The properties of bioengineered chondrocyte sheets for cartilage regeneration
Primer ID | Accesion No. | Sequence | Expect size(bp) |
|---|---|---|---|
Collagen Type 1-F | NM_000088 | AAG GGT GAG ACA GGC GAA CAA | 170 |
Collagen Type 1-R | TTG CCA GGA GAA CCA GCA AGA | ||
Collagen Type II-F | NM_033150 | GGA CTT TTC TTC CCT CTC T | 113 |
Collagen Type II-R | GAC CCG AAG GGT CTT ACA GGA | ||
SOX9-F | NM_000346 | AAC GCC GAG CTC AGC AAG A | 138 |
SOX9-R | CCG CGG CTG GTA CTT GTA ATC | ||
Collagen27α1-F | NM_032888 | GGG CCT TAT GGA AAT CCA GGT C | 176 |
Collagen27α1-R | GGT CCA GGA TAG CCC TTG TGT C | ||
Integrinα10-F | NM_003637 | CTG GGA TAT GTG CCC GTG TG | 112 |
Integrinα10-R | TTG GAG CCA TCC AAG ACA ATG A | ||
Fibronectin1-F | NM_001030524 | GCA CAG GGG AAG AAA AGG AG | 189 |
Fibronectin1-R | TTG AGT GGA TGG GAG GAG AG |