Figure 4

Immunohistochemical examination: fluorescence microscopy of layered chondrocyte sheets. Fibronectin (shown in green) was detected in the periphery of the triple-layered chondrocyte sheets (A, D). However, collagen type II (shown in green) was located in the pericellular matrix of the triple-layered chondrocyte sheets (B, E) and integrin α10 (shown in green) was scattered diffusely throughout the triple-layered chondrocyte sheets (C, F). The blue color shows counterstained DNA. DAPI excites at about 360 nm and emits at about 460 nm when bound to DNA, producing a blue fluorescence. A, B, C: Scale bar = 20 μm. D, E, F: Scale bar = 2 μm