From: Real-time quantification of wild-type contaminants in glyphosate tolerant soybean
Dilution levela | 1 | 2 | 3 | 4 | 5 | 6 | 7 |
---|---|---|---|---|---|---|---|
Expected wt DNA content (%) | 5.00 | 1.00 | 0.20 | 0.04 | 0.0080 | 0.0016 | 0.0008 |
Estimated wt genome copies (n.) | 8,889 | 1778 | 356 | 71 | 14.2 | 2.8 | 0.6 |
Signal rateb | 9/9 | 9/9 | 9/9 | 9/9 | 9/9 | 6/9 | 6/9 |
Mean Ct value of positivesc | 26.18 | 28.78 | 31.44 | 33.77 | 35.72 | 35.55 | 35.59 |
Mean CV of Ct values (%)d | 0.78 | 0.84 | 0.71 | 1.85 | 2.28 | - | - |
Estimated wt DNA content (%)e | 5.00 | 0.82 | 0.13 | 0.026 | 0.0067 | 0.0075 | 0.0074 |
Bias (%)f | 0 | -18 | -35 | -35 | -16 | 371 | 819 |