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Figure 1 | BMC Biotechnology

Figure 1

From: Real-time quantification of wild-type contaminants in glyphosate tolerant soybean

Figure 1

Development of a PCR assay specific for a transgenic event or its corresponding wt sequence at the insertion site. On the left, event-specific assay for testing AP of GMOs in conventional lots: from the known sequence of the insert a flanking plant DNA region is characterized and oligonucleotides are designed to detect the junction. On the right, wt-specific assay for testing trait purity in GM lots: from the characterized flanking region, the wt sequence allelic to the insert is obtained and oligonucleotides are designed to detect the reconstructed plant insertion site.

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