Figure 3

Immunofluorescence using multi-species antibodies. A: The scFv-Fc F2C (a-c), SF9 (d-f), TA10 (g-i) and AA2 (j-l) in their human (a, d, g, j), mouse (b, e, h, k) or rabbit (c, f, i, l) versions can be used in immunofluorescence to detect their respective target proteins with similar efficiency (red). Bar = 20 μm. B: Using the multi-species approach, antibodies can be produced fused to any of the three IgG species. This allows a large diversity of multiplexing. In this example, Myosin, Tubulin and Giantin were co-detected in HeLa cells using the human version of SF9 (a), the mouse version of F2C (b) and the rabbit version of TA10 (c) respectively. The three labelings are shown overlayed in (d). Bar = 20 μm.