Figure 2

Using scFv-Fc antibody for immunofluorescence. Cells were immuno-labeled using either scFv (a, c, e, g) or scFv-hFc (b, d f, h) antibodies. scFv were detected using an anti-Myc tag antibody followed by Cy3-labeled anti-mouse antibodies and scFv-hFc using Cy3-labeled anti-human antibodies (red). Nuclei were stained using DAPI (blue). AA2 (a, b), TA10 (c, d), SF9 (e, f) and F2C (g, h) detect respectively Rab6-GTP, Giantin, alpha-Tubulin and nonmuscle MyosinIIA. scFv fused to a hFc portion keep their specificity and are, in general, more sensitive and easier to use than their monomeric scFv counterparts. Bar = 20 μm.