Expression data of the d2 × 250 bp vectors. Flow cytometric data and corresponding determination of proviral load for d2 × 250 vectors in three different cell types: RN33B (naïve) (A-E), 293T (F-K) and K562 (L-Q). Cells were transduced at MOI 1 and 5 (relative DNA titer) and analysed 7 days after transduction. The figure shows the percentage of GFP positive cells for each vector (A, D, F, I, L and O) along with the corresponding mean fluorescence (MFU) (B, E, G, J, M and P) and proviral load (the latter only for selected vectors) (C, H, K, N and Q). Error bars denote standard deviations.