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Table 1 Summary of conditions and primers used for RT-PCR.

From: Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres

Genes

Primer Sequences (5'-3')

Cycles

Size (bp)

Annealing Temperature (°C)

Pluripotency-associated markers

Oct4

F

TCTCGCCCCCTCCAGGT

30

218

53

 

R

GCCCCACTCCAACCTGG

   

Sox2

F

GGCAGCTACAGCATGATGCAGGACC

34

130

60

 

R

CTGGTCATGGAGTTGTACTGCAGG

   

Endodermal-associated markers

FoxA2

F

GGGAGCGGTGAAGATGGA

38

89

55

 

R

TCATGTTGCTCACGGAGGAGTA

   

Gata6

F

GAGTGAGAGAAGATGGAAGGG

38

250

55

 

R

CGCACATGAAATCTGGATGTGG

   

Ectodermal-associated markers

Nestin

F

CAGCTGGCGCACCTCAAGATG

30

209

55

 

R

AGGGAAGTTGGGCTCAGGACTGG

   

Pax6

F

AACAGACACAGCCCTCACAAACA

30

275

55

 

R

CGGGAACTTGAACTGGAACTGAC

   

Mesodermal-associated markers

Brachyury

F

GAACCAGCACCCTGTGTCCAC

38

608

57

 

R

GCCACGACAAAAAGTCACTGC

   

Goosecoid

F

CTCAACCAGCTGCACTGTC

38

322

55

 

R

GTCAGCTGTCCGAGTCCAAATC

   

Control-associated markers

GAPDH

F

ATGGAGAAGGCTGGGGCTC

28

196

55

 

R

AAGTTGTCATGGATGACCTTG

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