From: Unrestricted somatic stem cells from human umbilical cord blood grow in serum-free medium as spheres
Genes | Primer Sequences (5'-3') | Cycles | Size (bp) | Annealing Temperature (°C) | |
---|---|---|---|---|---|
Pluripotency-associated markers | |||||
Oct4 | F | TCTCGCCCCCTCCAGGT | 30 | 218 | 53 |
 | R | GCCCCACTCCAACCTGG |  |  |  |
Sox2 | F | GGCAGCTACAGCATGATGCAGGACC | 34 | 130 | 60 |
 | R | CTGGTCATGGAGTTGTACTGCAGG |  |  |  |
Endodermal-associated markers | |||||
FoxA2 | F | GGGAGCGGTGAAGATGGA | 38 | 89 | 55 |
 | R | TCATGTTGCTCACGGAGGAGTA |  |  |  |
Gata6 | F | GAGTGAGAGAAGATGGAAGGG | 38 | 250 | 55 |
 | R | CGCACATGAAATCTGGATGTGG |  |  |  |
Ectodermal-associated markers | |||||
Nestin | F | CAGCTGGCGCACCTCAAGATG | 30 | 209 | 55 |
 | R | AGGGAAGTTGGGCTCAGGACTGG |  |  |  |
Pax6 | F | AACAGACACAGCCCTCACAAACA | 30 | 275 | 55 |
 | R | CGGGAACTTGAACTGGAACTGAC |  |  |  |
Mesodermal-associated markers | |||||
Brachyury | F | GAACCAGCACCCTGTGTCCAC | 38 | 608 | 57 |
 | R | GCCACGACAAAAAGTCACTGC |  |  |  |
Goosecoid | F | CTCAACCAGCTGCACTGTC | 38 | 322 | 55 |
 | R | GTCAGCTGTCCGAGTCCAAATC |  |  |  |
Control-associated markers | |||||
GAPDH | F | ATGGAGAAGGCTGGGGCTC | 28 | 196 | 55 |
 | R | AAGTTGTCATGGATGACCTTG |  |  |  |